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. Author manuscript; available in PMC: 2009 Jan 24.
Published in final edited form as: Stem Cells. 2008 Apr 10;26(6):1496–1505. doi: 10.1634/stemcells.2008-0102

Figure 4. Expression of downstream mediators of pluripotency.

Figure 4

(A.) Relative expression levels for transcripts identified by Palmqvist et al.28 as being closely correlated with functional pluripotency in low (yellow) and high (red) pass Eednull ES cells. Most genes are expressed at wild-type levels or higher in mutant ES cells. (B.) Real-time RT-PCR verification of selected genes represented in panel A. Real-time RT-PCR was carried out in duplicate on RNA generated from pooled cell samples (3 different samples per pool). Error bars for panel D represent standard error from 2 replicate pools.