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. Author manuscript; available in PMC: 2009 Jan 24.
Published in final edited form as: Oncogene. 2006 Feb 13;25(24):3436–3444. doi: 10.1038/sj.onc.1209366

Figure 5.

Figure 5

Suppression of MDM2 expression or direct Akt activity relieves Wnt-mediated suppression of hAR protein expression. (a) Western blot (top) shows that MDM2 protein expression is suppressed by greater than 88% by an siRNA that targets the gene and this siRNA relieves the Wnt-mediated suppression of hAR expression induced by transfection with β-catenin or PCDH-PC. (middle). Actin control (bottom). (b) Western blot shows that direct suppression of Akt signaling by inhibitor 5233705 but not by PI3-kinase inhibitor LY294002 relieves Wnt-mediated suppression of hAR protein (top) and Wnt-mediated upregulation in phosphorylation of MDM2 (middle) in β-catenin transfected LNCaP cells. Actin control (bottom).