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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Cell Signal. 2008 Oct 29;21(2):264–273. doi: 10.1016/j.cellsig.2008.10.014

Figure 1. GSK3 inhibitors reduce migration of microglia in vitro.

Figure 1

In the scratch migration assay, BV-2 microglia were preincubated for 30 min with GSK3 inhibitors 20 mM lithium (Li), 10 μM indirubin-3’-monoxime (Ind), or 10 μM kenpaullone (Ken), or with non-GSK3 kinase inhibitors 10 μM SB203580 and 10 μM D4476. The scratch was implemented and the cells were imaged immediately and after incubation for 6 hr. (A) Representative images at 6 hr after scratching (scale bar = 200 μm). (B) Wound closure was calculated by dividing widths measured after a 6 hr incubation by the initial scrape width. Values are Means ± SEM; n=3; the dashed line represents the average response with the three GSK3 inhibitors. *p<0.05 compared with control in the absence of GSK3 inhibitor.