Skip to main content
. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Cell Signal. 2008 Oct 29;21(2):264–273. doi: 10.1016/j.cellsig.2008.10.014

Figure 2. GSK3 inhibitors attenuate random and directed microglial migration in vitro.

Figure 2

(A) Random migration of BV-2 microglia to the bottom of a transwell migration chamber was measured after treatment with GSK3 inhibitors 20 mM lithium (Li), 10 μM SB216763 (SB21), 10 μM kenpaullone (Ken), or 10 μM indirubin-3’-monoxime (Ind) after a 6 hr incubation. (B) Chemotaxis of BV-2 microglia towards 50 μM CCL2 in the bottom chamber was measured with or without GSK3 inhibitors for 6 hr. The chemotactic gradient was abolished by incubating with CCL2 in the top and bottom (CCL2 t+b) chambers. Control values averaged 34 ± 5 cells per field of view, with 9 high-power fields counted per well. Values are Means ± SEM; n = 3 wells per group in at least 3 independent experiments; the dashed line represents the average response with the GSK3 inhibitors. *p<0.05 compared with untreated control cells; †p<0.05 compared with CCL2-treated cells in the absence of GSK3 inhibitor.