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. Author manuscript; available in PMC: 2010 Jan 1.
Published in final edited form as: Anal Biochem. 2008 Sep 18;384(1):34–41. doi: 10.1016/j.ab.2008.09.019

Fig. 2.

Fig. 2

Responsiveness of fluorescent indicators to Cu(I) (a) Cu(II) associates more rapidly and more tightly with FluoZin-3 than Cu(I). Cu(I) experiment in the presence of 1.5% Na2SO3 and Cu(II) in its absence. (b) Addition of Cu(I) or Cu(II) to FluoZin-3 under reducing conditions (1.5 %Na2SO3). (c) Response of NPG (500nM) to Cu(I) under different redox conditions. (d) Cu(I) is stabilized by the presence of Na2SO3. Black line, NPG is added prior to Cu(I) addition at 1 min. Dotted line, Cu(I) is added at time zero and the NPG is added at 15 min.