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. Author manuscript; available in PMC: 2009 Dec 31.
Published in final edited form as: Respir Physiol Neurobiol. 2008 Jul 31;164(3):300–311. doi: 10.1016/j.resp.2008.07.019

Figure 6.

Figure 6

Fluorescence photomicrographs of substance P (SP)-immunoreactive nerve cell bodies within superficial muscular plexus and SP-immunoreactive nerve fiber density within tracheal smooth muscle in IL-1 Ra or saline-treated tracheal segments after in vitro exposure to air or ozone. A (air exposure with IL-1 Ra pretreatment): few SP-immunoreactive nerve fibers are present in tracheal smooth muscle (NFD of this micrograph is 0.20). B (ozone exposure with saline pretreatment): increased SP-immunoreactive nerve fibers in tracheal smooth muscle (NFD of this micrograph is 0.41). C (ozone exposure with IL-1 Ra pretreatment) decreased SP-immunoreactive nerve fibers in tracheal smooth muscle (NFD of this micrograph is 0.21). D (air exposure with IL-1 Ra pretreatment): negative SP-immunoreactive neurons are present in the SMP. E (ozone exposure with saline pretreatment): SP-immunoreactive cell bodies are increased in the SMP. F (ozone exposure with IL-1 Ra pretreatment): negative SP-immunoreactive neurons are seen. The images of the SP-containing nerve cell bodies in SMP and SP NFD in air exposure group with saline pretreatment (not shown) are the same as air exposure with IL-1 pretreatment. Magnification: x285.