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. Author manuscript; available in PMC: 2009 Dec 30.
Published in final edited form as: Biochemistry. 2008 Dec 30;47(52):13842–13849. doi: 10.1021/bi801430e

Figure 4. Structural effects of Q513 mutations in the dark and lit states monitored by limited proteolysis.

Figure 4

Limited proteolysis by chymotrypsin digestion was performed at room temperature for the wildtype protein and Q513 mutants in both the dark (left panel) and lit states (right panel). Time points ranging from 0 to 60 min are shown above the gels. The molecular weight marker indicating 14 kDa and 20 kDa are also shown on the left side of the gels. Black filled circles represent the full-length undigested domain and open circles represent the largest stable digested product.