Table 2.
Reversal Potential (Erev) and Permeability Ratios (PX/PCl−) for CASTOR and castor-2
| CASTOR
|
castor-2
|
|||
|---|---|---|---|---|
| Ion (X) | Erev (mV) | PX/PCl− | Erev (mV) | PX/PCl− |
| K+ | 36 ± 2 (n = 40) | 6.0a | 28 ± 2 (n = 48) | 3.7a |
| Na+ | 11 ± 1 (n = 8) | 1.6a | 25 ± 3 (n = 13) | 3.2a |
| Ca2+ | −29 ± 2 (n = 13) | 0.5b | −32 ± 2 (n = 19) | 0.6b |
a
Permeability ratios calculated from reversal potentials using equation Erev = RT/zF × ln(PK+[K]o/PNa+[Na]i).
b
Permeability ratios calculated from reversal potentials using equation Erev= RT/F × ln(√((4PCa2+[Ca]o/PK+[K]i)+1/4) − 1/2), where RT/F = 25.26 mV and Z = 1. The junction potential correction was as follows: NaCl (250 mM/20 mM, agar bridge, 2 M KCl), Vm = Vcmd + 2.3 mV; KCl (250 mM/20 mM, agar bridge, 2 M KCl), Vm = Vcmd + 1.2 mV; CaCl2 (125 mM/10 mM, agar bridge, 2 M KCl), Vm = Vcmd + 3.1 mV.