Figure 1.
Quantification of Insoluble and Soluble Glucans in Wild-Type and DBE Mutant Plants.
Plants were harvested and immediately frozen in liquid N2. Each sample comprised all of the leaves from a single plant (A) or complete individual rosettes ([B] and [C]). Total insoluble and soluble glucans were extracted using perchloric acid, and glucans were measured after enzymatic hydrolysis to glucose. FW, fresh weight.
(A) Total insoluble (top) and soluble (bottom) glucans from wild-type and DBE mutant plants harvested at the end of a 12-h photoperiod (white bars) or at the end of the night (black bars). Each value is the mean ± se of eight replicate samples. Note the change of scale on the y axis.
(B) Total insoluble glucan and phytoglycogen precipitated from the soluble fraction of isa1/isa2 (white squares), isa1/isa2/isa3 (black squares), isa1/isa3 (red circles, no line), and isa2/isa3 (blue circles, no line). Each point is the mean ± se from four replicate samples. Wild-type plants grown in parallel had 7.4 ± 0.2 and 0.6 ± 0.0 mg starch/g fresh weight at the end of the day and the end of the night, respectively.
(C) Total insoluble glucan and phytoglycogen precipitated from the soluble fraction of the isa1/isa2/isa3/lda quadruple mutant (gray diamonds). Additional data are given for isa1/isa3/lda (green triangles, no line) and isa2/isa3/lda (gold triangles, no line). Each point is the mean ± se from four replicate samples.