Figure 3.

Glucans Accumulating in Leaf Palisade and Adjacent Epidermal Cell Plastids of the Wild Type, the isa1/isa2 Double Mutant, and the isa1/isa2/isa3/lda Quadruple Mutant, Visualized by Transmission Electron Microscopy.

Starch granules (s) were present in wild-type plastids and in the epidermal cell plastids of isa1/isa2. No starch granules were visible in the quadruple DBE mutant. Soluble glucan and/or particulate material (black arrowheads) are indicated in isa1/isa2 mesophyll cells and in the quadruple mutant. Note the absence of glucans from wild-type and isa1/isa2 palisade cell chloroplasts at the end of the night (white arrowheads) but the glucan particles remaining in the quadruple DBE mutant. Additional images from these and other mutants are provided in Supplemental Figure 7 online. pal, leaf palisade cell plastid; epi, epidermal cell plastid. Bars = 2 μm.