Figure 4.

Scanning Electron Micrographs of Insoluble Glucans Isolated from the Wild Type, the isa1/isa2 Double Mutant, and the Quadruple DBE Mutant.

(A) Starch granule from the wild type.

(B) Starch granules isolated from the isa1/isa2 double mutant.

(C) Nanoscale insoluble glucan particles isolated from the isa1/isa2 double mutant.

(D) Nanoscale insoluble glucan particles isolated from the quadruple DBE mutant.

(E) As in (D), at higher magnification.

(F) As in (E), after incubation of the particles in water at 95°C for 10 min. No particles similar to those in (D) and (E) were visible.

Black bars = 500 nm; white bars = 200 nm.