Figure 9.

Glucans Accumulating in Plastids of Leaf Cells of the Quintuple Mutant Lacking All Four DBEs and the Endoamylase AMY3, Visualized by Transmission Electron Microscopy.

Starch granules (s) and soluble glucans (black arrowheads) were observed in the plastids of all cell types examined, although there was cell-to-cell variation within the same section. For example, some bundle sheath cells and epidermal cells (epi) appeared to contain only starch granules (shown), whereas others contained soluble glucan or a mixture (not shown). Palisade cell (pal) chloroplasts contained predominantly soluble glucan (most similar in appearance to the material in isa1/isa2 mesophyll chloroplasts) and infrequent small starch granules. Bars = 2 μm.