Figure 5.

Gm PNC1 Is Abundant during Postgerminative Growth.

The homogenates of etiolated soybean cotyledons grown for 1, 3, 5, 7, 9, 11, 13, and 15 d in the dark were prepared. Green cotyledons were obtained from seedlings grown for 8 d in the dark followed by 1, 3, 5, and 7 d in light were also analyzed. Gm PNC1, ICL, and GO were detected by immunoblotting using the antibodies indicated on the left. ICL and GO are markers for glyoxysomal and leaf peroxisomal proteins, respectively. Total protein obtained from an equal number of cotyledons was contained in the lanes of the gel.