FIG. 5.

Gene regulatory representations from Giant-regulated lacZ reporter gene. To generate gene regulatory plots, data derived from slices (lacZ mRNA channel [A] and Giant protein channel [B]) are plotted in a 2D space. Only two slices are shown for clarity. The plots numbered 1–6 show how robust lacZ levels present in ventral portions of the embryo are repressed in regions containing peak Giant levels such as in plot 1–4. In more dorsally located regions (plot 5–6), limiting levels of activators reduce lacZ expression regardless of Giant levels. The overlaid lines, obtained by fitting the data with a rational function, show the trend of the relation between levels of Giant and lacZ.