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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: J Immunol. 2008 Nov 15;181(10):7055–7061. doi: 10.4049/jimmunol.181.10.7055

Figure 4.

Figure 4

A non-phosphorylatable form of LAX retains the ability to inhibit TCR/CD28-induced activation of RE/AP. Jurkat T cells were transfected with 10 μg of either an (A) RE/AP or (B) NFAT luciferase reporter along with 3μg of either wt LAX or LAX 4YF expression plasmids (and 7 μg empty vector), or with 10μg of either LAX construct, or with 10 μg vector only (V). The following day, cells were left unstimulated or stimulated with anti-TCR/CD28 antibodies for 7 hours prior to assaying luciferase expression. Data is shown as fold activation relative to unstimulated samples. Error bars reflect SEM from two independent experiments. (C) An anti-myc western blot is also presented to show equivalent expression of LAX WT and LAX 4YF in the Jurkat lysates used for luciferase assays above.