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. Author manuscript; available in PMC: 2009 Nov 15.
Published in final edited form as: J Immunol. 2008 Nov 15;181(10):7055–7061. doi: 10.4049/jimmunol.181.10.7055

Figure 5.

Figure 5

The association of ALX with LAX maps to two distinct site within the LAX cytoplasmic tail. (A) C-terminal truncation mutants of LAX are shown, with the four tyrosines in the cytoplasmic tail of LAX that are sites of TCR-induces tyrosine phosphorylation denoted. (B-D) Expression plasmids for myc-tagged wt LAX or the LAX truncations shown in fig. 5A were co-transfected into 293T cells with YFP-tagged ALX constructs. WT ALX (B), ALX C (C), or ALX ΔC (D) proteins were used. Each experiment was otherwise performed as in Fig. 3. Note that the LAX truncations were expressed at a higher level than WT LAX as shown in the whole cell extract controls, and any increase in association with ALX appears proportional to the increased expression.