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The region of LAX between the D3 and D4 truncations is critical for inhibition of both (A) RE/AP and (B) NFAT reporters. Jurkat T cells were transfected as in Fig. 4, using LAX truncations shown in Fig. 5A. Error bars reflect SEM from three independent experiments. (C) An anti-myc western blot is also presented to show expression of LAX in the Jurkat lysates used for luciferase assays above.
