Skip to main content
. Author manuscript; available in PMC: 2009 May 21.
Published in final edited form as: Mol Cell. 2008 Nov 21;32(4):491–502. doi: 10.1016/j.molcel.2008.10.016

Figure 1. Altered methylation on H3K27 in Ezh2−/− cells.

Figure 1

(A) Targeting strategy to generate Ezh2 conditional and knockout alleles. Exon number is indicated above each exon shown in boxes. Lines represent introns.

(B–C) Western blot analysis of nuclear extracts (B) and histones (C) isolated from wild-type (J1 and CJ7) and mutant (Ezh2−/− and Eed−/−) ES cells.

(D) ChIP-qPCR analysis of H3K27me3, EZH2 and SUZ12 binding at promoters of PcG target genes in wild-type (WT), Ezh2−/− and Eed−/− ES cells. The y-axis shows relative enrichment of ChIP’ed DNA versus the inputs. Actb promoter and an intergenetic region (Cs) serve as negative controls.