Table 1.
ANK2 Exon Organization
| Exon # | Previous exon # | 5′ splice | 3′ splice | Length (bp) | 3′ intron (bp) | Domain | Comments |
|---|---|---|---|---|---|---|---|
| 0 | TCCCA/gt | 86190 | non-coding | ||||
| 1′ | ag/GTAAG | AAAAG/gt | 60/21* | 269901 | MBD | alt. start | |
| 1 | 1 | AGAAG/gt | 84* | 124603 | MBD | start | |
| 2 | 2 | ag/TCTGA | ATCAG/gt | 102 | 21850 | MBD | ANK repeat 1 |
| 3 | 3 | ag/AATGG | CTAAG/gt | 99 | 2544 | MBD | ANK repeat 2 |
| 4 | 4 | ag/AAGGG | CTCAG/gt | 99 | 33051 | MBD | ANK repeat 3 |
| 5 | 5 | ag/AATGG | CAGAG/gt | 99 | 4727 | MBD | ANK repeat 4 |
| 6 | 6 | ag/GATGG | CCAAG/gt | 186 | 426 | MBD | ANK repeats 5 & 6 |
| 7 | 7 | ag/ATGAT | CTGAG/gt | 24 | 2862 | MBD | |
| 8 | 8 | ag/AGTGG | CCAGG/gt | 99 | 1527 | MBD | ANK repeat 7 |
| 9 | 9 | ag/AATGG | CTAGG/gt | 99 | 7554 | MBD | ANK repeat 8 |
| 10 | 10 | ag/GATGG | CTAAG/gt | 99 | 5872 | MBD | ANK repeat 9 |
| 11 | 11 | ag/AATGG | CCCTG/gt | 198 | 2117 | MBD | ANK repeats 10 & 11 |
| 12 | 12 | ag/AATGG | CAGAG/gt | 99 | 164 | MBD | ANK repeat 12 |
| 13 | 13 | ag/TCTGG | ACATT/gt | 99 | 6485 | MBD | ANK repeat 13 |
| 14 | 14 | ag/CGTGG | CCAGG/gt | 99 | 9456 | MBD | ANK repeat 14 |
| 15 | 15 | ag/GAGGA | CCAAG/gt | 198 | 3187 | MBD | ANK repeats 15 & 16 |
| 16 | 16 | ag/AAGGG | GGAAG/gt | 99 | 524 | MBD | ANK repeat 17 |
| 17 | 17 | ag/AACGG | CCAAG/gt | 99 | 4116 | MBD | ANK repeat 18 |
| 18 | 18 | ag/AATGG | CTAAG/gt | 198 | 4732 | MBD | ANK repeats 19 & 20 |
| 19 | 19 | ag/AGTGG | CAAAG/gt | 99 | 684 | MBD | ANK repeat 21 |
| 20 | 20 | ag/CTTGG | CCAAG/gt | 99 | 3929 | MBD | ANK repeat 22 |
| 21 | 21 | ag/AACGG | CTGCG/gt | 99 | 925 | MBD | ANK repeat 23 |
| 22 | 22 | ag/AATGG | CCACA/gt | 99 | 9228 | MBD | ANK repeat 24 |
| 23 | 23 | ag/ACTAT | AGAGG/gt | 73 | 1384 | MBD | |
| 24 | ag/CTCTT | TTCAG/gt | 54 | 6977 | MBD | ||
| 25 | 24 | ag/GTGAT | GACAG/gt | 145 | 6307 | SBD | |
| 26a | 25 | ag/CCTTC | ACCAG/gt | 103 | 707 | SBD | |
| 26b | ag/TTCCG | ACCAG/gt | 88 | 707 | SBD | alt. 3′ splice site | |
| 27 | 26 | ag/GTGTC | TCAGG/gt | 104 | 5138 | SBD | |
| 28 | ag/CCGCG | AGCAG/gt | 36 | 6451 | SBD | ||
| 29 | 27 | ag/TTTCC | CTTGG/gt | 225 | 1501 | SBD | Zu-5 motif |
| 30 | 28 | ag/TAAAC | CTTGG/gt | 99 | 983 | SBD | |
| 31 | 29 | ag/GCCTG | TGAAG/gt | 155 | 2637 | SBD | Zu-5 motif |
| 32 | 30 | ag/TACTG | TGCAG/gt | 212 | 519 | SBD | Zu-5 motif |
| 33 | 31 | ag/GCTCA | AACAG/gt | 205 | 2444 | SBD | |
| 34 | 32 | ag/GTGGA | GCCAG/gt | 97 | 2365 | SBD | |
| 35 | 33 | ag/GTTCT | TGGAG/gt | 229 | 1100 | SBD | |
| 36 | 34 | ag/GTGTT | TCAAG/gt | 126 | 2757 | SBD | |
| 37 | 35 | ag/GTACG | CAAAG/gt | 123 | 2253 | SBD | |
| 38 | 36 | ag/GAATC | AAGAG/gt | 33 | 1919 | SBD | |
| 39 | 37 | ag/ATCGA | AAAAA/gt | 22 | 2795 | SBD | |
| 40 | 38 | ag/ATGAT | TGAAG/gt | 6255 | 1523 | 220 kD insert | |
| 41 | 39 | ag/ATCCA | GACAG/gt | 75 | 2440 | DD | |
| 42 | 40 | ag/AATTA | TACAG/gt | 132 | 1569 | DD | |
| 43 | 41 | ag/ATACC | TGAAG/gt | 144 | 2383 | DD | |
| 44 | 42 | ag/GGTTC | TATTT/gt | 286 | 1662 | CTD | |
| 45 | 43 | ag/TGTGA | AAAAG/gt | 292 | 2727 | CTD | |
| 46 | ag/GAGCT | TTCAG/gt | 93 | 464 | CTD | ||
| 47 | 44 | ag/GGAGA | AGAAG/gt | 84 | 111 | CTD | α-helix |
| 48 | 45 | ag/GTTAC | CAGAG/gt | 165 | 1314 | CTD | α-helix |
| 49 | ag/GTCAC | AAGAG/gt | 183 | 3143 | CTD | ||
| 50 | ag/GCTTT | AAAAG/gt | 92 | 3275 | CTD | ||
| 51a | 46 | ag/GACAA | 15** | CTD | stop | ||
| 51b | ag/GACAA | 184** | CTD | alt. stop |
Fields represent: new and previous exon numbering, 5′- and 3′-splice sites, exon length (bp), proceeding intron length (bp), corresponding functional domain, and additional comments. For exon 1′ and 1, the length is measured from the beginning of the open reading frame and is denoted with an asterisk. Exon 26a/b represents alternative 3′-splice acceptor sites that allow for the addition or subtraction of 15 bp. The Zu-5 motif constitutes the minimal binding domain for β-spectrin [3]. Exon 40 encodes the 220 kD insert that is found in brain-specific 440 kD ankyrin-B [29]. Exon 51a/b represents alternative stop sites depending on the open reading frame. The terminal amino acid sequence for 51a is “DNNE”, while the terminal sequence for 51b is “QEAK”. Length of exons 51a/b corresponds to the distances to the in-frame stop codons and is marked with double asterisks.