(A) HBMEC were transfected with a FasL siRNA or a scrambled siRNA sequence (control) and examined after 72 hr for the presence of FasL in the cell lysate by Western blot. A quantitative analysis of the data obtained by the scanning of 3 separate blots is shown in the lower panel. The data are the mean ratios (± SD) of FasL/actin.
(B) HBMEC were transfected with PAI-1 siRNA, FasL siRNA or a combination of both siRNAs and their respective scrambled controls and examined after 72 hr for apoptosis by flow cytometry. The data represent the mean numbers (± SD) of apoptotic cells from triplicate samples (* p<0.025, ** p<0.01, # p<0.005).
(C) Cell lysates of HBMEC treated as indicated in the figure were examined for PARP cleavage by Western blot analysis.