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. Author manuscript; available in PMC: 2009 Oct 7.
Published in final edited form as: Cancer Cell. 2008 Oct 7;14(4):324–334. doi: 10.1016/j.ccr.2008.08.012

Figure 5. FasL is Required for EC Apoptosis upon PAI-1 Downregulation.

Figure 5

(A) HBMEC were transfected with a FasL siRNA or a scrambled siRNA sequence (control) and examined after 72 hr for the presence of FasL in the cell lysate by Western blot. A quantitative analysis of the data obtained by the scanning of 3 separate blots is shown in the lower panel. The data are the mean ratios (± SD) of FasL/actin.

(B) HBMEC were transfected with PAI-1 siRNA, FasL siRNA or a combination of both siRNAs and their respective scrambled controls and examined after 72 hr for apoptosis by flow cytometry. The data represent the mean numbers (± SD) of apoptotic cells from triplicate samples (* p<0.025, ** p<0.01, # p<0.005).

(C) Cell lysates of HBMEC treated as indicated in the figure were examined for PARP cleavage by Western blot analysis.