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. Author manuscript; available in PMC: 2009 Dec 10.
Published in final edited form as: Neuron. 2008 Dec 10;60(5):832–845. doi: 10.1016/j.neuron.2008.09.037

Figure 6. FKBP12 cKO mice display enhanced contextual associative fear memory, altered novelty interaction, and increased perseverative/repetitive behavior.

Figure 6

(a) Mean freezing behavior of wild type (WT) and FKBP12 cKO (KO) mice. Both genotypes performed similarly during training and had similar freezing behavior in both STM contextual and cued memory tests. FKBP12 KO mice display enhanced freezing during LTM contextual fear memory testing but no difference in cued associative fear memory (* p>.05, One way ANOVA). (b) FKBP12 cKO mice display enhanced contextual LTM in the absence of STM testing (* p=<.05, One way ANOVA). (c) mTORC1 levels increase in wild-type mice following fear conditioning. mTOR immunoprecipate complexes isolated from untreated mice (naïve), training environment exposure only (con), and fear conditioned (context+shock, FC) and allowed to recover 15 or 60 min (FC 15’ or FC 60’). mTORC1 levels compared to naïve wild-type control. Naïve levels: KO, 179 ± 21%. Context: WT, 156 ± 28%; KO, 208 ± 30%. FC 15’: WT, 172 ± 23%; KO: 214 ± 31%. FC 60’: WT, 104 ± 9%; KO: 176 ± 18% (* p<.05, ** p<.01, ANOVA) WT n=5, KO n=4 for each time point. (d) FKBP12 cKO mice display greater repetitive behavior as determined by marble burying during 30-min test period. Marbles buried, WT: 10.9 ± 1.9; KO: 15.3 ± 0.9 (* p<.05, ANOVA) (e) FKBP12 cKO mice display preference for the familiar object during object recognition analysis. Dashed line represents PI=0.5, or neutral preference. WT mice, n=11; KO, n=10 (*, + p<0.05, ++ p<.01, ANOVA).