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. 2009 Feb;174(2):361–370. doi: 10.2353/ajpath.2009.080521

Figure 2.

Figure 2

Confirmation of functional expression of HB-NPM-ALK in GP293. A: Western blot analysis showed expression of NPM-ALK in the pcDNA-NPM-ALK and HB-NPM-ALK transfected GP293 cell line but not in the empty vector transfected GP293 cell line. Expression of functional NPM-ALK proteins was examined by using anti-pStat3 antibody. Western blot analysis probed with anti-pStat3 showed that in the transfectant, Stat3 was phosphorylated. B: Comparison of NPM-ALK transfected GP293 cells with ALK+ALCL cells, demonstrating that similar levels of NPM-ALK were expressed.