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. 2009 Feb;174(2):390–400. doi: 10.2353/ajpath.2009.080513

Figure 9.

Figure 9

Effects of bone marrow-derived fibroblast-conditioned media on lung fibroblast procollagen I and α-smooth muscle actin gene expression. Conditioned media were prepared from purified GFP-positive/CD11b-negative fibroblasts from lungs of CRA-challenged mice, and added to media of cultured GFP-negative lung fibroblasts. The effects of this treatment with conditioned media (GFP− + CM) on procollagen I (A, C) and α-smooth muscle actin (B) mRNA levels were compared with the levels in untreated GFP-positive (GFP+) and GFP-negative (GFP−) cells. Data are expressed as 2−ΔΔCT, using GAPDH as the reference and the GFP-positive cell signal as calibrator. A and B: Significance (#P < 0.05) compared with mean signal in GFP-positive cells. Significance compared with mean signal in untreated GFP-negative cells. C: Significance (*P < 0.05) compared with mean signal in fibro + aged media samples. Significance compared with mean signal in fibro + CM + IgG samples. N = 10 animals per group.