Figure 5.

Asbestos-induced c-Jun phosphorylation is inhibited in C10 lung epithelial cells transfected with siPKD. Cells were homogenized, and Western blot analyses were performed using antibodies to p-c-Jun, total c-Jun, and β-Actin as described in Materials and Methods. A: Western blots of representative time course study. B: Quantitation of data. C: Representative Western blots after using the siPKD construct or a scrambled control construct (sicontrol) D: Quantitation of assays using siPKD or sicontrol transfected C10 cells. Results are typical of three independent experiments. *P ≤ 0.05 in comparison with unexposed cells (0), ^†P ≤ 0.05 in comparison with sicontrol group at the same time. Bars = Mean ± SEM.