Figure 1.

Cav-3 is normally expressed in the myo-epithelial cell layer in the mammary gland. Mammary glands were harvested from 2-month-old wild-type virgin female mice. Paraffin-embedded sections were prepared and immunostained with anti-Cav-3 IgG (brown color). Then, samples were counterstained with hematoxylin (blue color). Note that Cav-3 is preferentially expressed in the myo-epithelial cell layer (arrows). A negative control (omission of the primary antibody) is also shown. Scale bar = 50 μm.