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. 2009 Feb;174(2):630–637. doi: 10.2353/ajpath.2009.080566

Figure 3.

Figure 3

Fetal cells mainly express endothelial markers in mouse B16-BL6 melanoma during pregnancy. A: Photomicrographs show a fetal cell expressing the endothelial marker von Willebrand factor in the maternal tumor; A1: EGFP fluorescence (green) combined with DAPI counterstain (blue); A2: von Willebrand factor staining (red) combined with DAPI counterstain; A3: merge. B: Photomicrographs show fetal cells expressing the endothelial marker von Willebrand factor in blood vessels locating in periphery of the maternal tumor. B1: EGFP fluorescence combined with DAPI counterstain. B2: The fetal EGFP+ cells are localized in the walls of blood vessels and express von Willebrand factor (white arrow). B3: These EGFP+ cells are homogenously Y chromosome-positive (green) in nuclei with DAPI counterstain (white arrow) suggesting that they derive from a common progenitor. C: Photomicrographs show fetal cells expressing the LYVE-1 marker in lymphatic vessels of the maternal tumor. C1: EGFP fluorescence combined with DAPI counterstain; C2: LYVE-1 staining combined with DAPI counterstain; C3: merge. The fetal EGFP+ cells are localized in the walls of lymphatic vessels and display LYVE-1 antigen (white arrow). D: Photomicrographs showing a fetal leukocyte in the maternal tumor. D1: EGFP fluorescence combined with DAPI counterstain; D2: CD45 staining combined with DAPI counterstain; D3: merge, an EGFP+ cell in the tumor. Adjacent to this fetal cell (white arrow), two maternal CD45+ cells can be visualized as well (yellow arrows). Original magnifications: ×1000 (A3); ×400 (B3, C, D2, D3).