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. 2009 Feb;174(2):647–660. doi: 10.2353/ajpath.2009.080685

Figure 5.

Figure 5

Tetraspanins CD9 and CD151 are not required for the motility-inhibitory activity of CD82. A: The expression of tetraspanins in HT1080 and Du145 cells. Top: tetraspanin CD9, CD82, and CD151 proteins were analyzed using the cell lysates generated from HT1080-Mock, HT1080-CD82, Du145-Mock/Control shRNA, Du145-CD82/Control shRNA, Du145-Mock/CD151 shRNA, and Du145-CD82/CD151 shRNA transfectants by Western blot. The β1 integrin proteins were used as a protein loading control. Bottom: the flow cytometry analysis of CD151 expression in Du145-Mock and -CD82 cells that stably express control and CD151 shRNA. Black line: negative control mAb mouse IgG; gray line: CD151 mAb 5C11. B: The inhibition of cell migration by CD82 is independent of CD151. The Transwell migration onto FN (10 μg/ml) and LN1 (10 μg/ml) was measured in Du145-Mock/CD151 shRNA and -CD82/CD151 shRNA transfectants as described above. The results represent the mean ± SE number of three independent experiments; P = 0.01 between the Mock/CD151 shRNA and CD82/CD151 shRNA transfectant cells on FN and <0.01 on LN1.