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. Author manuscript; available in PMC: 2009 Dec 30.
Published in final edited form as: Biochemistry. 2008 Dec 30;47(52):13942–13951. doi: 10.1021/bi8015789

Figure 6.

Figure 6

Experimental (circles) and calculated (solid lines) 15N{31P} REDOR dephasing (Δ/S0) as a function of dipolar evolution time for sidechain nitrogens (highlighted in red) of S. cerevisiae lumazine synthase complexed with the 6-carbon-linker phosphonate 16. The experimental dephasing was the result of the accumulation of 1,613,824 scans for both S and S0 REDOR spectra. The dashed line for the upper right plot indicates that the maximum dephasing of the arginine peak is 0.055, which corresponds to 38% occupancy of the lumazine synthase active sites. The dashed line for the lower right plot shows that the corresponding expected maximum dephasing of the lysine peak is 0.034 (from 0.38/11). The observed dephasing maximum of the lysine peak is 0.013.