Table 1.
Distances between Phosphorus of the Ligand and Nitrogen Atoms of the Protein in S. cerevisiae Lumazine Synthase
| X-ray distances (1EJB) 5-carbon linker compound 15 (Å)a | REDOR distances for 5-carbon linker compound 15 (Å)b | REDOR distances for 6-carbon linker compound 16 (Å)c | |
|---|---|---|---|
| P to NZ of Lys92 | 7.4 | 6.2 | 3.8 |
| P to NE of Arg136 | 3.8 | 4.5 | 4.0 |
| P to NH of Arg136 | 5.6 | 3.8 | 3.8 |
| P to NH of Arg136 | 3.8 | 3.8 | 3.9 |
| P to NE of His148 | 6.8 | 5.4 | 4.7 |
| P to NE of His97 | 7.2 | 4.1 | 4.7 |
a
The distances were obtained from the published crystal structure of S. cerevisiae lumazine synthase in complex with the 5-carbon-linker compound 15 (22).
b
The distances were calculated from the REDOR NMR dephasing displayed in Figure 5.
c
The distances were calculated from the REDOR NMR dephasing displayed in Figure 6.