TABLE 1.
Summary of the ligand-screening test in silico and ex vivoa
| Method | Parameter | Number | Hit rate (%)b |
|---|---|---|---|
| Compounds tested in silico | Compounds with a binding score of less than −11.45 kcal/mol | 590 | |
| High-ranking and available compounds (out of 590) | 205 | ||
| Compounds tested ex vivo | Cytotoxic compound | 1 | |
| Not effective (relative PrPSc level of >70%) | 180 | ||
| Effective (relative PrPSc level of <70%) | 24 | 11.7 | |
| More effective (relative PrPSc level of <50%) | 4 | 2.0 |
a
The numbers of compounds (total n = ∼600,000) in in silico and ex vivo tests are summarized.
b
The hit rate was calculated from 205 compounds tested ex vivo.