TABLE 2.
Gene | Primer description | Primer sequence (5′→3′) | Product size (bp) |
---|---|---|---|
GAPDHa | Forward | GAA GTA CAC GGT GGA GGC TG | 206 |
Reverse | CGC TGA TCA CGA CCT TCT TC | ||
LdMTb | Forward | CAA GTG CCT TTC CAC CAG AAT C | 228 |
Reverse | CTC ACC TTT TTG AAC TCC AAC AGG | ||
LdRos3c | Forward | ACG ACA CGG CTT GAT TTT CG | 238 |
Reverse | GAG TAG TCC ACG GAG GCA GTA AAG |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
LdMT, L. donovani putative miltefosine transporter.
LdRos3, β-subunit of LdMT.