TABLE 1.
Primer sequences used for PCR amplification and sequencing
| Gene | Primer | Sequence (5′ to 3′) | Annealing temp (°C) | Reference |
|---|---|---|---|---|
| 16S rRNAa | pA | AGAGTTTGATCCTGGCTCAG | 62 | 11 |
| pH | AAGGAGGTGATCCAGCCGCA | |||
| sul1 | sul1bF | CTTCGATGAGAGCCGGCGGC | 63 | 29 |
| sul1bR | GCAAGGCGGAAACCCGCGCC | |||
| sul2 | sul2F | TCGTCAACATAACCTCGGACAG | 60 | V. Enne |
| sul2R | GTTGCGTTTGATACCGGCAC | |||
| sul3 | sul3F | GAGCAAGATTTTTGGAATCG | 51 | 18 |
| sul3R | CATCTGCAGCTAACCTAGGGCTTTGGA | |||
| intI1 | intA | ACAGGGCAAGCTTAGTAAAGCC | 67 | 22 |
| intB | CTCGCTAGAACTTTTGGAAA | |||
| intI2 | int2F | CACGGATATGCGACAAAAAGGT | 58.5 | 32 |
| int2R | GTAGCAAACGAGTGACGAAATG | |||
| qacE | KazamF1 | GGGAATTCGCCCTACACAACAAATTGGGAGA | 50 | 14 |
| KazamR1 | TACTCGAGTTAGTGGGCACTTGCTTTGG | |||
| qacEΔ1 | KazamF2 | GGGAATTCGCCCTACACAACAAATTGGGAGA | 60 | 14 |
| KazamR2 | GCTGCAGCTGCGGTACCACTGCCACAA |
a
16S rRNA gene sequence from bp 8 to 1522.