. 2008 Dec 8;53(2):696–702. doi: 10.1128/AAC.00652-07

TABLE 4.

Characterization of a number of cultured bacterial isolates encoding different sul genotypes

Isolate	Bacterial source^a	16S rRNA gene identification	% BLAST^b similarity	sul gene	Class of integron^c	Antibiotic resistance phenotype of isolates^e	MIC of SCP (mg liter⁻¹)
C237	1, Preapp (soil)	`Bacillus sphaericus`	98	1		Sm^r Tmp^r Nal^r	1
C422	2,21 (soil)	`Stenotrophomonas maltophilia`	98	1	2	Sm^r	1
C131	2,240 (soil)	`Pseudomonas fluorescens`	97	1		Tet^r	32
C3	Pig slurry	`Acinetobacter lwoffi`	99	1	2	NG	1
PGS22	Pig slurry	`Aerococcus viridians`	99	2	1^d	Sm^r Cm^r Tet^r Nm^r Nal^r	8
C506	Soil leachate	`Shigella flexneri`	99	2	1^d	Sm^r Cm^r Tet^r Tmp^r Nm^r Nal^r Km^r Amp^r	8
C701	1, Preapp (soil)	`Pseudomonas lini`	99	2	2	Sm^r Amp^r Tmp^r Nal^r	1
C439	2,90 (Soil)	`Acinetobacter sp. N2`	97	2	2	Sm^r Tet^r Tmp^r Nal^r	16
C5	Pig slurry	`Pseudomonas borealis`	99	3	2	Cm^r Tet^r	32
PGS48	Pig slurry	`Enterococcus hirae`	97	3		Sm^r Tet^r	4
C167	2,1 (soil)	`Providencia stuart2`	97	3		Sm^r Tet^r	6
C2	Pig slurry	`Acinetobacter lwoffi`	97	3		Sm^r Cm^r Tet^r Tmp^r Nal^r	5
C231	1,21 (soil)	`Pseudomonas putida`	97	1 + 2	1^d	Amp^r Cm^r Tet^r Nm^r Nal^r	8
C361	1,289 (soil)	`Arthrobacter arilaitensis`	99	1 + 2	1^d	Sm^r Cm^r Tet^r Nm^r Nal^r	5
PGS49	Pig slurry	`Acinetobacter sp. An9`	99	1 + 2	2	Sm^r Nal^r	16
C410	1,1 (soil)	`Psychrobacter ikaite`	98	1 + 2	2	Tet^r	16
PGS47	Pig slurry	`Acinetobacter lwoffi`	97	2 + 3	2	Sm^r Nal^r	16
PGS61	Pig slurry	`Enterococcus sulfureus`	97	2 + 3		Sm^r Tet^r	4
PGS21	Pig slurry	`Aerococcus viridans`	99	2 + 3	2	Cm^r Tet^r	32
C15	Pig slurry	`Acinetobacter lwoffi`	99	1 + 2 + 3	2	Sm^r Cm^r Tet^r Tmp^r Nm^r Nal^r	5
C20	Pig slurry	`Psychrobacter ikaite`	98	1 + 2 + 3		Sm^r Cm^r Tet^r Tmp^r Nal^r	5
C711	1, Preapp (soil)	`Psychrobacter sp. DY9-2`	97	1 + 2 + 3		Sm^r Tet^r Tmp^r Nal^r	8
C712	1, Preapp (soil)	`Psychrobacter frigidicola`	96	1 + 2 + 3		Sm^r Tet^r Tmp^r Nal^r	8
C713	1, Preapp (soil)	`Psychrobacter ikaite`	97	1 + 2 + 3	2	Sm^r Cm^r Tet^r Tmp^r Nal^r	8
C35	1, Preapp (soil)	`Acinetobacter calcoaceticus`	97	1 + 2 + 3		Sm^r Cm^r Tet^r Tmp^r Nal^r	8
C36	1, Preapp (soil)	`Acinetobacter calcoaceticus`	97	1 + 2 + 3		Sm^r Cm^r Tet^r Tmp^r Nal^r	8
C37	1, Preapp (soil)	`Acinetobacter lwoffi`	97	1 + 2 + 3		Sm^r Cm^r Tet^r Tmp^r Nal^r	8
C44	1,1 (soil)	`Acinetobacter rhizosphaerae`	98	1 + 2 + 3		Sm^r Cm^r Tet^r Tmp^r Nm^r Nal^r	5
C141	2,21 (soil)	`Acinetobacter lwoffi`	98	1 + 2 + 3		Sm^r Km^r Cm^r Tet^r Tmp^rNm^r Nal^r Amp^r	6
C328	2,21 (soil)	`Bacillus psychrodurans`	98	1 + 2 + 3		Sm^r Km^r Cm^r Tet^r Nm^r Nal^r	8
C442	2,90 (soil)	`Acinetobacter baumannii`	99	1 + 2 + 3		Cm^r Tet^r Nal^r	8

1, Preapp (soil), soil cores from year 1 before slurry application; 1,1, soil cores from year 1, day 1 time point; 1,289, soil cores from year 1, day 289 time point; 2,1, soil cores from year 2, day 1 time point.

Percent nucleotide similarity of an approximately 800-bp 16S rRNA gene sequence to bacterial strains submitted to databases and searched using the BLAST program (1).

qacEΔ1/qacE was screened for in all intI1-positive isolates.

qacEΔ1 detected.

Antibiotic resistance breakpoints: Sm^r, resistance to 16 μg ml⁻¹ streptomycin; Amp^r, 16 μg ml⁻¹ ampicillin; Km^r, 16 μg ml⁻¹ kanamycin; Cm^r, 16 μg ml⁻¹ chloramphenicol; Tet^r, 8 μg ml⁻¹ tetracycline; Tmp^r, 16 μg ml⁻¹, trimethoprim; Nm^r, 8 μg ml⁻¹ neomycin; Nal^r, 16 μg ml⁻¹, nalidixic acid. NG, no growth.