TABLE 4.
Effect of EA and its analogues on transcripts and activities of selected redox enzymes in A549 cells after 24 h of treatmenta
| Inhibitor and concn (μM) | TrxR
|
GST
|
GR
|
GPx
|
||||
|---|---|---|---|---|---|---|---|---|
| qRT-PCRb | Enzyme activityc | qRT-PCR | Enzyme activity | qRT-PCR | Enzyme activity | qRT-PCR | Enzyme activity | |
| EA | ||||||||
| 166 | 115 | 82 ± 7 | 122 | 109 ± 7 | 152 | 87 ± 11 | 109 | 86 ± 18 |
| 662 | 131 | 77 ± 9 | 131 | 101 ± 7 | 125 | 85 ± 15 | 150 | 125 ± 20 |
| FEA | ||||||||
| 158 | 97 | 91 ± 12 | 115 | 99 ± 8 | 105 | 87 ± 12 | 107 | 122 ± 22 |
| 315 | 61 | 115 ± 18 | 164 | 121 ± 12 | 70 | 119 ± 16 | 77 | 112 ± 19 |
| CEA | ||||||||
| 5 | 76 | 42 ± 10 | 68 | 101 ± 6 | 77 | 94 ± 6 | 51 | 85 ± 15 |
| 24 | 73 | 45 ± 9 | 84 | 106 ± 9 | 84 | 103 ± 8 | 71 | 78 ± 17 |
a
All values are given as percentages of the values for untreated controls.
b
All values given for quantitative real-time PCR (qRT-PCR) in this table were normalized for GAPDH. However, for all transcripts the same trends were observed when normalizing for 18S rRNA (data not shown).
c
TrxR activity in cell extracts was measured with DTNB as the substrate.