TABLE 4.
Expression of qnr alleles
| Strain and plasmid | Inducing agent | Relative RNA transcript level ata:
|
|
|---|---|---|---|
| 15 min | 30 min | ||
| J53 (lexA+recA+) | |||
| pMG298 (qnrB1) | Ciprofloxacin | 3.7 | 3.2 |
| Mitomycin C | 2.6 | 2.8 | |
| pMG301 (qnrB2) | Ciprofloxacin | 5.9 | |
| pMG317 (qnrB3) | Ciprofloxacin | 2.1 | |
| pMG319 (qnrB4) | Ciprofloxacin | 9.9 | 9.0 |
| Mitomycin C | 8.2 | 7.9 | |
| pMG252 (qnrA1) | Ciprofloxacin | 0.9-1.0 | |
| Mitomycin C | 0.9-1.5 | ||
| AB1157 (lexA+recA+) | |||
| pMG319 (qnrB4) | Ciprofloxacin | 3.9 | |
| Mitomycin C | 2.0 | ||
| AB1157 LexA300::spec (lexA[Def] recA+) | |||
| pMG319 (qnrB4) | Ciprofloxacin | 1.1 | |
| Mitomycin C | 1.0 | ||
| DM49 (lexA3[Ind] recA+) | |||
| pMG319 (qnrB4) | Ciprofloxacinb | 1.2 | |
| Mitomycin C | 1.0 | ||
a
All measurements were repeated at least twice.
b
The ciprofloxacin MIC of DM49 pMG319 was 0.064 μg/ml, compared to the MIC of of 0.25 μg/ml for AB1157 pMG319 and AB1157 LexA300::spec pMG319, and so a ciprofloxacin concentration of 0.05 μg/ml was used for induction.