FIG. 5.

Inhibition of gp46 binding to cellular receptors by polyanions. Soluble recombinant gp46-Fc fusion protein or negative control soluble Fc protein was preincubated with compounds before application to detached HeLa cells. After 2 h of incubation, cells were washed to remove unbound gp46-Fc or Fc control protein. Cells were stained with an anti-Fc phycoerythrin-conjugated antibody and analyzed by flow cytometry. Results are expressed as the percent inhibition of gp46-Fc binding and are from one representative experiment performed in triplicate, and error bars represent ±1 SD.