TABLE 2.
Summary of ID90 and ID50 values
| Measurement and assay | Valuea for:
|
||||
|---|---|---|---|---|---|
| PRO 2000 | D2S | DexS | Pcr-400 | HTLV-1 sera | |
| ID90 | |||||
| Syncytium formation | 12.7 ± 2.2 | NAb | 3.4 ± 1.7 | 1.2 ± 0.3 | NA |
| gp46-Fc binding | 4.2 ± 0.5 | 5.9 ± 3.0 | 4.7 ± 0.9 | NDc | ND |
| Pseudovirus transduction | 11.5 ± 2.5 | 11.5 ± 3.2 | 8.4 ± 5.8 | 0.5 ± 0.1 | 0.00001 ± 0.0 |
| ID50 | |||||
| Syncytium formation | 0.12 ± 0.1 | 28.0 ± 8.1 | 1.83 ± 1.5 | 0.15 ± 0.1 | 0.015 ± 0.07 |
| gp46-Fc binding | 0.14 ± 0.15 | 0.01 ± 0.0 | 0.16 ± 0.1 | ND | ND |
| Pseudovirus transduction | 4.7 ± 0.6 | 9.2 ± 2.9 | 0.8 ± 0.1 | 0.04 ± 0.0 | 0.0005 ± 0.0 |
a
ID50 and ID90 values were calculated from the results of at least two independent replicate experiments and are the means ± SEM, with the exceptions of gp46-Fc binding assay values and the ID50 of D2S determined in the syncytium formation assay, which are the means ± SD of results from one experiment performed in triplicate, and the values for HTLV-1 sera, which are mean reciprocals of dilution ± SEM from independent replicate experiments. Values for all agents other than HTLV-1 sera are expressed in micromolars.
b
NA (not applicable) indicates that 90% inhibition was not obtained.
c
ND, not determined.