FIG. 5.
Comparison of the catalytic efficiencies of HCV NS3/4A variants in HeLa cells coexpressing the sensor (ECFP-CSwt-citrine). The ratio of sensor/protease expression plasmid DNA used was 1:9 to ensure that most cells expressing the sensor had protease expression as well. (A) Each point of the scatter plot represents the Eapp of a single cell. The results for the experimental samples were compared to those for the control (wt −; sensor without scNS3/4A) by using one-way ANOVA with Dunnett's posttest. **, P < 0.01; ns, not significant; SD, standard deviation. (B) Western blot of the cytosolic extracts of transfected cells. The bands corresponding to full-size fusion protein (56.7 kDa) and its cleavage products (28.3 kDa) are indicated. wt/−, control.