TABLE 2.

Oligonucleotide primers used in study

Target(s)	Sequence (5′-3′)a	Start point (bp)b	Amplicon size (bp)b	GenBank accession no.c
TEM	F: 5′-GTGCGCGGAACCCCTATT	107	968	AB194682
	R: 5′-TTACCAATGCTTAATCAGTGAGGC	1075
SHV	F: 5′-CTTTACTCGCCTTTATCGGC	174	982	AF124984
	R: 5′-TTACCGACCGGCATCTTTCC	1156
CTX-M-2 and -5	F: 5′-ATGTGCAGYACCAGTAAGG	292	564	X92506
	R: 5′-TAAGTGACCAGAATMAGCGG	856
CTX-M	F: 5′-ATGTGCAGYACCAGTAAAG	292	562	X92506
	R: 5′-GGTCACCAGAAGGAGC	854
OXA-1	F: 5′-CAGATTCAACTTTCAAGATCG	1564	612	J02967
	R: 5′-GTGTTTAGAATGGTGATCG	2176
OXA-2 and -20	F: 5′-GCRTCSACATTCAAGATWCC	332	524	X03037
	R: 5′-TCWTCCATYCTGTTTGGCG	856
OXA-5 and -10	F: 5′-AGCATCAACATTYAARATYCC	1855	597	AF347074
	R: 5′-ATGATGCCYTCACTTKCC	2452
PER-1 and -2	F: 5′-GGCCTGACGATCTGGAACC	1841	855	AY866517
	R: 5′-TAACTGCATAACCTACTCC	2696
ACT-1	F: 5′-ACAGGCAAGCAGTGGCAGG	379	619	U58495
	R: 5′-GGATTCACTTCTCTCGCAGGC	998
MIR-1d,e	F: 5′-TCGGTAAAGCCGATGTTGCG	1115	301	M37839
	R: 5′-CTTCCACTGCGGCTGCC	1416
CMY and LAT	F: 5′-ATTCCGGGTATGGCCGT	133	835	EF406116
	R: 5′-GGGTTTACCTCAACGGC	968
FOX-1 to -5	F: 5′-GACGGCATTATCCAGCCG	800	856	X77455
	R: 5′-GTAACCGGATTGGCCTGGAAGC	1656
DHA-1 and -2	F: 5′-ATCTGCAACACTGATTTCCG	1001	1,115	Y16410
	R: 5′-GCACTCAAAATAGCCTGTGC	2116
ACC-1 and -2e	F: 5′-AACAGCCTCAGCAGCCGG	253	342	EF554600
	R: 5′-GCCGCAATCATCCCTAGC	595
KPC-1 to -3	F: 5′-ATGTCACTGTATCGCCGTCT	131	892	AF297554
	R: 5′-TTTTCAGAGCCTTACTGCCC	1023
16S rRNA	F: 5′-GCCAGCAGCCGCGGTAATACG	467	289	M87484
	R: 5′-GGACTACCAGGGTATCTAATCC	756

^aIUB codes (underlined) are as follows: K, G+T; M, A+C; R, A+G; S, G+C; W, A+T; Y, C+T. F, forward; R, reverse.

^bThe numbering indicates the 5′ nucleotide of each primer and is according to the sequence referenced.

^cwww.ncbi.nlm.nih.gov.

^dThis primer set also amplifies ACT-1.

^ePrimers modified from those described previously (36).