TABLE 1.
MICs of peptide-AcpP11 PMO conjugates in pure cultures of E. coli
| Motif and PMO no. | Conjugated peptide | MIC
|
|
|---|---|---|---|
| μM | μg/ml | ||
| Motif 1 (C-N-N) | |||
| 05-0198 | RFFXB | >80 | >360 |
| 05-0197 | RFFRFFXB | 20 | 99 |
| 05-0200 | RFFRFFRFFRXB | 2.5 | 14 |
| 05-0653 | dRFdFdRdFdFdRdFdFdRXBa | 5 | 28 |
| 23-248 | RXXRXXRXXB | 20 | 102 |
| 06-0199 | KFFKFFKFFKXB | 10 | 54 |
| 06-0064 | RBBRBBRBBRXB | >80 | >408 |
| 06-0079 | RVVRVVRVVRXB | >80 | >422 |
| 06-0082 | OFFOFFOFFOXB | >80 | >431 |
| Motif 2 (C-N) | |||
| 06-0067 | RXRXB | >80 | >358 |
| 06-0070 | RXRXRXRXB | 80 | 401 |
| 06-0073 | RXRXRXRXRXRXB | 1.25 | 6.9 |
| 06-0761 | KXKXKXKXKXKXB | >80 | >430 |
| 06-0762 | OXOXOXOXOXOXB | >80 | >424 |
| Motif 3 (C-N-C) | |||
| 06-0076 | RXRRXRRXRRXRXB | 1.25 | 7.2 |
| 06-0824 | KXKKXKKXKKXKXB | 20 | 111 |
| 06-0763 | OXOOXOOXOOXOXB | 10 | 54 |
| 07-0795 | RFRRFRRFRRFRXB | 0.625 | 3.7 |
| 07-1119 | RXRRBRRXRRBRXB | 2.5 | 14 |
| Control (ampicillin) | 6.7 | 2.5 | |
a
d, the residue that follows is of the d-isomeric form.