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. 2008 Nov 17;29(3):736–744. doi: 10.1128/MCB.01313-08

FIG. 8.

FIG. 8.

Appearance of Ikbkap−/− mice with a human wild-type IKBKAP transgene. (A) The arrowheads point to Ikbkap null mice with the human wild-type IKBKAP transgene. No significant phenotypic differences were observed compared to heterozygous littermates. (B) PCR genotyping results of genomic DNA from tail snips with dedicated primers. Lane 1 (from left), Ikbkap+/−genotype; lanes 2 and 3, Ikbkap1−/− genotype. The mice represented by lanes 2 and 3 were positive for carrying the human transgene. wt, wild-type fragment; ko, knockout fragment; TG, human IKBKAP transgene. (C) Western blot result using the human-specific IKAP antibody to confirm the presence of the human IKBKAP protein, IKAP, in mouse brains from different genotypes. Lane 1, positive control of nuclear extracts from HeLa cells, which are positive for IKAP expression; lane 2, control mouse (no human IKBKAP [WT] transgene); lane 3, Ikbkap+/+ mouse with human WT transgene; lane 4, Ikbkap−/− mouse with human WT transgene; lane 5, Ikbkap−/− mouse with human FD transgene. Arrowhead 150-kDa position.