TABLE 1.
Saccharomyces cerevisiae strains
| Straina | Genotype | Reference |
|---|---|---|
| JRY19 | MATahis4 leu2 trp1 ura3 | 22 |
| JRY2234 | MATaade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 (W303-1A) | 54 |
| JRY3009 | MATα ade2-1 his3-11,15 leu2-3,112 trp1-1 ura3-1 can1-100 (W303-1B) | |
| CFY345 | JRY3009 HMR-SSa | 19 |
| CFY762 | JRY3009 HMR-SSasir1Δ::LEU2 | 22 |
| CFY932b | MATatrp1-901 leu2-3,112 ura3-52 his3-200 gal4Δ gal80Δ GAL2-ADE2 LYS2::GAL1-HIS3 met2::GAL7-lacZ sir2Δ::TRP1 | 33 |
| CFY770 | JRY3009 HMR-SSa (ACS GAL4 ABF1) sir1Δ::LEU2 | 22 |
| CFY1463 | JRY3009 HMR-SSaSIR1-HA3-kanMX6 | 4 |
| CFY1487 | JRY3009 HMR-SSasir1R493G-HA3-kanMX6 | 4 |
| CFY1535 | JRY3009 HMR-SSasir1n1-HA3-kanMX6 | |
| CFY1538 | JRY3009 HMR-SSasir1n2-HA3-kanMX6 | |
| CFY1583 | JRY3009 HMR-SSasir1NΔ-HA3-kanMX6 | |
| CFY1804 | JRY3009 HMR-SSasir3Δ::URA3 | |
| CFY2079 | JRY3009 HMR-SSasir3BAHΔ | |
| CFY2665 | JRY3009 HMR-SSasir1D36A-HA3-kanMX6 | |
| CFY2664 | JRY3009 HMR-SSasir1W38A-HA3-kanMX6 | |
| CFY2666 | JRY3009 HMR-SSasir1Q27A,N29A-HA3-kanMX6 |
a
All strains except JRY19 and CFY932 are isogenic derivatives of W303.
b
CFY932 is a sir2Δ::TRP1 derivative of the two-hybrid strain described in reference 33.