TABLE 2.
Plasmids
| Plasmid | Description | Reference |
|---|---|---|
| pTT65 | GAD-Orc1(5-228) | 55 |
| pCF413 | pADH1-GBD-SIR1 in pRS426 | 22 |
| pCF409 | pADH1-GBD-SIR1 in pRS416 | |
| pCF415 | pADH1-GBD-sir1V490D in pRS426 | 51 |
| pCF1520 | pADH1-GBD-sir1n1 in pRS426 | |
| pCF1522 | pADH1-GBD-sir1n2 in pRS426 | 22 |
| pCF721 | pADH1-GBD-SIR1(473-678) in pRS426 | |
| pCF1879 | pADH1-GBD-sir1D36A in pRS426 | |
| pCF1878 | pADH1-GBD-sir1W38A in pRS426 | |
| pCF1880 | pADH1-GBD-sir1Q27A,N29A in pRS426 | |
| pCF1881 | pADH1-NLS-SIR1OIR in pRS426 | |
| pCF1883 | pADH1 in pRS426 | |
| pCF1618 | pADH1-GAD-SIR3BAH | |
| pCF1861 | pADH1-SIR1(25-155)-GBD | |
| pCF91 | pMET3 in pRS313 | 18 |
| pCF1917 | pMET3-SIR1 in pRS313 | |
| pCF1916 | GST-ORC1(5-219) in pGEX-KG | 55 |
| pCF1748 | ORC1(5-219) in pET28b | 30 |
| pCF1586 | SIR1(480-611)C593A in pET28b | 30 |
| pCF1808 | SIR1(25-678)L26V in pET28b |