TABLE 1.
Primers used for cloning
| Primer | Orientationa | Sequenceb | Description |
|---|---|---|---|
| pCMVmyc-SUMO1 | F | ATCCCC[AAGCTT]ATGTCTGACCAGGAGGCA | HindIII |
| R | ATTCGC[GGATCC][CTA]ACCCCCCGTTTGTTCCTG | BamHI; stop codon | |
| pCMVmyc-SUMO2 | F | AATCCG[GAATTC]ATGGCCGACGAAAAGCCCAA | EcoRI |
| R | TAACGC[GGATCC][TCA]ACCTCCCGTCTGCTGTTGG | BamHI; stop codon | |
| pCMVmyc-SUMO3 | F | AATCCG[GAATTC]ATGTCCGAGGAGAAGCCC | EcoRI |
| R | AATCGC[GGATCC][CTA]ACCTCCCGTCTGCTGCTG | BamHI | |
| pCMV myc-Ufm1 | F | CACCCG[GAATTC]ATGTCGAAGGTTTCCTTTAAG | EcoRI |
| R | ATTCGC[GGATCC]TTAACAACTTCCAACACGATC | BamHI | |
| pGEX4T-1 N-CTCF (amino acids 1-266) | F | TACCG[GAATTC]ATGGAAGGTGAGGCGGTTG | EcoRI |
| R | TATCCG[CTCGAG]CTGGAATGTTTTCTTTACACC | XhoI | |
| pGEX4T-1 C-CTCF (amino acids 570-736) | F | TTACGC[GGATCC]ATGGCAAGACATGCAGATAACTG | BamHI |
| R | TATCCG[CTCGAG]TCACCGGTCCATCATGCTGAG | XhoI |
a
F, forward; R, reverse.
b
Brackets indicate restriction enzyme sites and stop codons engineered into the primers.