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SUMOylated SF-1 exhibits WT nuclear localization. Nuclear (N) and cytoplasmic (C) fractions of Y1 cells (1 × 106) stably expressing WT HIS-FLAG SF-1, K194R SF-1, or HA-SUMO2-K194R SF-1 were subjected to anti-FLAG, anti-HA-11, anti-SF-1, anti-lamin, and anti-tubulin immunoblotting (IB).
