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. Author manuscript; available in PMC: 2009 Jan 26.
Published in final edited form as: Cell. 2007 Mar 9;128(5):915–929. doi: 10.1016/j.cell.2007.01.031

Figure 5. Depletion of Coronin 1B alters lamellipodial dynamics and inhibits SSH1L-induced membrane ruffling.

Figure 5

A- Depletion of Coronin 1B inhibits SSH1L-induced hyper-ruffling. Rat2 fibroblasts stably expressing SSH1L or FP4-CAAX were transfected with the Coronin 1B shRNA, and the cells were subjected to kymography analysis as described in Fig. 1E. Newman-Keuls multiple comparison test was used after one-way ANOVA to generate the P values.

B- Cofilin S3D suppresses SSH1L-induced hyper-ruffling. Phospho-mimetic mutants of either Coronin 1B (S2D) or Cofilin (S3D) were transiently expressed in Rat2 fibroblasts stably expressing SSH1L-GFP; kymography analysis was carried out as in Fig. 1E.