Table. Diagnosis of Acanthamoeba spp. and mycobacterial infection*.

Organism, patient specimen	Microscopy	PCR	Culture	Immunostaining	Serology
Acanthamoeba spp.
Cerebrospinal fluid	Negative	Positive	Negative	NA	NA
Brain biopsy	Negative	Positive	Negative	Negative	NA
Bronchial secretions	Negative	Positive	Negative	NA	NA
Lung biopsy	Negative	Positive	Negative	Negative	NA
Skin biopsy	Negative	Positive	Negative	NA	NA
Blood	NA	NA	NA	NA	Positive
Mycobacterium tuberculosis
Cerebrospinal fluid	Positive	Positive	Positive	NA	NA
Brain operative specimen	Positive	Positive	Negative	NA	NA
Sputum	Negative	Positive	NA	NA	NA
Bronchial secretions	Negative	Positive	Positive	NA	NA
Lung biopsy	Negative	Negative	Negative	NA	NA
Blood	NA	Positive	Negative	NA	NA

*NA, not available.