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Expression of Nox4 in siRNA treated RASMCs. RASMCs were treated with scrambled (Scr) or Nox4 siRNA (sinox4) for five days and then stimulated with 100 nM AngII for 4-hours. Expression of Nox4 was measured by real-time PCR (A) and Western Blot analysis (B). Data are average from five separate experiments ± Standard Error. Western Blot shows typical Western blot of Nox4 expression in scrambled or Nox4 siRNA treated RASMCs.
