Figure 3. WAT mRNA and protein levels.
Gene expression in adipose tissue from mice treated with DT for 9 days was analyzed by measuring relative mRNA levels using qPCR for a) the macrophage marker F4/80, b) the pro-inflammatory cytokine IL-6, c) the pro-inflammatory chemokine and macrophage chemoattractant MCP-1, and d) the anti-inflammatory cytokine IL-10. The protein levels of e) MCP-1 and f) IL-10 were measured in adipose tissues lysates of HFD-fed WT and CD11c-DTR BMT mice. Data are expressed as mean ± SEM. *P<0.05; **P<0.01; ***P<0.001; ns means not significant. n ≥ 4 per group.