Figure 5.

Expression patterns of V-ATPase subunit B2 isoform in reproductive ducts and accessory glands. Real-time qPCR results show gene expression profiles for the B2 subunit of vacuolar-type proton pump V-ATPase (Atp6v1b2) across a 24-h period in 3 parts of epididymis (caput, corpus, cauda), vas deferens, prostate, and seminal vesicles. Data are efficiency corrected, normalized, relative gene-expression values for the mean of reaction triplicates. Total RNAs were obtained from the same individuals sacrificed for investigating clock gene and Car2 expression (see Figs. 234). Horizontal bars represent day (open) and night (filled), with ZT indicated below.